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KMID : 0545120070170020313
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Journal of Microbiology and Biotechnology
2007 Volume.17 No. 2 p.313 ~ p.319
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Statistical Optimization of Medium Components for the Production of Biosurfactant by Bacillus licheniformis K51
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Joshi
Sanket
Yadav Sanjay
Nerurkar Anuradha
Desai Anjana J.
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Abstract
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The nutritional medium requirement for biosurfactant production by Bacillus licheniformis K51 was optimized. The important medium components, identified by the initial screening method of Plackett-Burman, were H3PO4, CaCl2, H3BO3, and Na-EDTA. Box-Behnken response surface methodology was applied to further optimize biosurfactant production. The optimal concentrations for higher production of biosurfactants were (g/l): glucose, 1.1; NaNO3, 4.4; MgSO4·7H2O, 0.8; KCl, 0.4; CaCl2, 0.27; H3PO4, 1.0 ml/l; and trace elements (mg/l): H3BO3, 0.25; CuSO4, 0.6; MnSO4, 2.2; Na2MoO4, 0.5; ZnSO4, 6.0; FeSO4, 8.0; CoCl2, 1.0; and Na-EDTA, 30.0. Using this statistical optimization method, the relative biosurfactant yield as critical micelle dilution (CMD) was increased from 10× to 105×, which is ten times higher than the non-optimized rich medium.
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KEYWORD
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Bacillus licheniformis, biosurfactant, Plackettburman, Box-Behnken, critical micelle dilution (CMD)
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